@VTuber_Lily This just reminded me that Birdo is canonically trans. In the 80s, the Mario 2 booklet said she was "a he who think he's a she" or something awful, but ever since then she has just been referred to as a "she."
Probably, this was just Nintendo realizing the original "joke" was not funny and pretty out of place in a children's game, but I choose to see it as a parent coming to terms with their trans child: first awkward and in denial, then accepting and loving.